skip to Main Content

Dicarbonyls induce senescence of human vascular endothelial cells.


Maximus Peto’s Commentary

This was a cell culture study, so its results may not be representative of what happens in aging humans, but it seems relevant. Could GO and MGO contribute to an accumulation of senescent endothelial cells during human aging, which may more-or-less permanently effect the function of the endothelium, assuming senescent cells accumulate and do not revert to quiescent (non-senescent) cells? If so, could these senescent cells be expected to be ablated with senescent cell ablation methods? Would that fix the problems that senescent endothelial cells presumably cause?


Dicarbonyls induce senescence of human vascular endothelial cells.
Mech Ageing Dev. 2017 Sep;166:24-32.
Navarrete Santos A, Jacobs K, Simm A, Glaubitz N, Horstkorte R, Hofmann B
DOI: 10.1016/j.mad.2017.07.009
PubMed publication date (edat): 8/7/2017

Abstract

RATIONALE:
Glyoxal (GO) and Methylglyoxal (MGO) are two dicarbonyls involved in the formation of advanced glycation end products (AGEs). Endothelial cells in the vessels are in constant contact with circulating AGEs and dicarbonyls. With this project, we aimed to elucidate the effect of GO and MGO on primary human vascular endothelial cells (HVECs).

METHODS:
Graft material from patients with coronary heart disease was used as HVECs source. HVECs were treated with different concentrations of GO and MGO. β-Galactosidase related senescence activity and cell morphology were analyzed. AGEs as well as p21 protein expression, glyoxalase-I expression and oxidative stress were detected.

RESULTS:
We here provide evidences that GO and MGO induce senescence in primary HVECs. Mechanistically GO and MGO induce senescence by increasing the ROS production, the expression of p21, the accumulation of AGEs and the arrest of HVECs in the G2 cell cycle phase. Aminoguanidine – a dicarbonyl scavenger – abrogated the effect of GO and MGO.

CONCLUSION:
Our data are relevant as they suggest that in diseases with elevated dicarbonyl concentrations, deleterious effects on the endothelium and the development of vascular dysfunction have to be expected. On the other hand, treatment of patients with dicarbonyl scavenger could prevent this.

PMID: 28780382
Free Full-Text:

Maximus Peto

Max Peto is a longevity researcher and founder of Long Life Labs. A biochemist by training, he studies the biochemistry of aging and longevity and has worked with research organizations such as SENS Research Foundation, Methuselah Foundation, BioAge Labs, Life Extension Foundation, and Ichor Therapeutics. His work at Long Life Labs is focused on empowering people to understand and manage the most critical factors for better health and longer life.

Free report on high blood pressure

The American Heart Association estimates more than 100 million Americans have high blood pressure, also known as “hypertension”. Learn more about the cause of high blood pressure and how you can reverse it in our free report.

Coming soon. We’ll send you the report as soon as it’s published.

*We do not share your email address with anyone.

Free longevity biomarker report

Biomarker levels predict the risk of early death—and we can change them! Learn about some important longevity biomarkers in our free report.

Coming soon. We’ll send you the report as soon as it’s published.

*We do not share your email address with anyone.

Free diabetes report

An estimated 50% of American adults have either prediabetes or type 2 diabetes. Learn more about the cause of type 2 diabetes, prediabetes, insulin resistance, and how to reverse them in our free report.

Coming soon. We’ll send you the report as soon as it’s published.

*We do not share your email address with anyone.

Back To Top