Maximus Peto’s Commentary
This looks like a potentially useful advancement: this group reports about their creation and characterization of a full-thickness human skin model, designed for testing “anti-aging compounds”. I wonder whether it can be glycated reliably…
Characterization of a New Reconstructed Full Thickness Skin Model, T-Skin™, and its Application for Investigations of Anti-Aging Compounds.
Int J Mol Sci. 2019 May 7;20(9). pii: E2240.
Michel Bataillon, Damien Lelièvre, Adeline Chapuis, Fabienne Thillou, Jean Baptiste Autourde, Steven Durand, Nathalie Boyera, Anne-Sophie Rigaudeau, Isabelle Besné, Christian Pellevoisin
PubMed publication date (edat): 5/10/2019
We have characterized a new reconstructed full-thickness skin model, T-Skin™, compared to normal human skin (NHS) and evaluated its use in testing anti-aging compounds.
The structure and layer-specific markers were compared with NHS using histological and immunohistological staining. In anti-aging experiments, T-SkinTM was exposed to retinol (10 µM) or vitamin C (200 µM) for 5 days, followed by immunohistological staining evaluation.
T-Skin™ exhibits a well stratified, differentiated and self-renewing epidermis with a dermal compartment of functional fibroblasts. Epidermal (cytokeratin 10, transglutaminase 1), dermo-epidermal junction (DEJ) (laminin 5, collagen-IV, collagen VII) and dermally-located (fibrillin 1, procollagen I) biomarkers were similar to those in NHS. Treatment of T-Skin™ with retinol decreased the expression of differentiation markers, cytokeratin 10 and transglutaminase 1 and increased the proliferation marker, Ki67, in epidermis basal-layer cells. Vitamin C increased the expression of DEJ components, collagen IV and VII and dermal procollagen 1.
T-Skin™ exhibits structural and biomarker location characteristics similar to NHS. Responses of T-Skin™ to retinol and vitamin C treatment were consistent with those of their known anti-aging effects. T-Skin™ is a promising model to investigate responses of epidermal, DEJ and dermal regions to new skin anti-ageing compounds.
Free Full-Text: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6540298/