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Characterization of a New Reconstructed Full Thickness Skin Model, T-Skin™, and its Application for Investigations of Anti-Aging Compounds.


Maximus Peto’s Commentary

This looks like a potentially useful advancement: this group reports about their creation and characterization of a full-thickness human skin model, designed for testing “anti-aging compounds”. I wonder whether it can be glycated reliably…


Characterization of a New Reconstructed Full Thickness Skin Model, T-Skin™, and its Application for Investigations of Anti-Aging Compounds.
Int J Mol Sci. 2019 May 7;20(9). pii: E2240.
Michel Bataillon, Damien Lelièvre, Adeline Chapuis, Fabienne Thillou, Jean Baptiste Autourde, Steven Durand, Nathalie Boyera, Anne-Sophie Rigaudeau, Isabelle Besné, Christian Pellevoisin
DOI: 10.3390/ijms20092240
PubMed publication date (edat): 5/10/2019

Abstract

BACKGROUND:
We have characterized a new reconstructed full-thickness skin model, T-Skin™, compared to normal human skin (NHS) and evaluated its use in testing anti-aging compounds.

METHODS:
The structure and layer-specific markers were compared with NHS using histological and immunohistological staining. In anti-aging experiments, T-SkinTM was exposed to retinol (10 µM) or vitamin C (200 µM) for 5 days, followed by immunohistological staining evaluation.

RESULTS:
T-Skin™ exhibits a well stratified, differentiated and self-renewing epidermis with a dermal compartment of functional fibroblasts. Epidermal (cytokeratin 10, transglutaminase 1), dermo-epidermal junction (DEJ) (laminin 5, collagen-IV, collagen VII) and dermally-located (fibrillin 1, procollagen I) biomarkers were similar to those in NHS. Treatment of T-Skin™ with retinol decreased the expression of differentiation markers, cytokeratin 10 and transglutaminase 1 and increased the proliferation marker, Ki67, in epidermis basal-layer cells. Vitamin C increased the expression of DEJ components, collagen IV and VII and dermal procollagen 1.

CONCLUSIONS:
T-Skin™ exhibits structural and biomarker location characteristics similar to NHS. Responses of T-Skin™ to retinol and vitamin C treatment were consistent with those of their known anti-aging effects. T-Skin™ is a promising model to investigate responses of epidermal, DEJ and dermal regions to new skin anti-ageing compounds.

PMID: 31067675
Free Full-Text: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6540298/

Maximus Peto

Max Peto is a longevity researcher and founder of Long Life Labs. A biochemist by training, he studies the biochemistry of aging and longevity and has worked with research organizations such as SENS Research Foundation, Methuselah Foundation, BioAge Labs, Life Extension Foundation, and Ichor Therapeutics. His work at Long Life Labs is focused on empowering people to understand and manage the most critical factors for better health and longer life.

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